Characterization of a Chitinase from Apocheima cinerarius nucleopolyhedrovirus

摘要：

　　Baculoviruses are important biological control agents against insect pests.In this work,a chitinase from Apocheima cinerarius nucleopolyhedrovirus (ApciNPV) was expressed to high levels in Escherichia coli and purified by affinity chromatography.The three-dimensional structure prediction of ApciNPV chitinase indicated the protein has an N-terminal polycystic kidney 1(PKD1) domain for chitin-substrate feeding and α/β TIM barrel catalytic domain characteristic of a family 18 glycohydrolase.

关键词： Apocheima cinerarius nucleopolyhedrovirus Chitinase Recombinant protein Insecticidal activity

作者: Qinghua Wang Yuxia Hou Liangjian Qu Zhilin Zhang Yuzhu Wang Yongan Zhang

作者单位: The key laboratory of forest protection,State forestry administration of china,Research institute of College of Science,China Agricultural University,Beijing,China Wulanchabu Forest Disease and Pest Control and Quarantine Station,ulanchabu,China

会议类型: 国内会议

会议名称: 第三届中国林业学术大会

会议地点: 福州

会议语种:英文

页码: 3-21

在线出版日期: 2013-09-01（万方平台首次上网日期，不代表论文的发表时间）