The influence of Bushenhuoxue on the podocytes of focal segmental glomerulosclerosis mice
OBJECTIVES: To observe the effects and mechanisms of Bushenhuoxue on desmin and nephrin expression in mice podocytes, and to investigate its effects on wtl expression in Wilms” tumor.METHODS: Adriamycin (ADR) was used to induce focal segmental glomerulous sclerosis (FSGS) in mice.Bushenhuoxue was used to treat FSGS for 6 weeks.We measured body mass and right renal mass, and determined serum albumin (ALB)levels, protein content in urine, and urinary protein and albumin creatinine ratio(UACR).Changes in renal tissue morphology were evaluated by microscopy.wtl and nepbrin expression in podocytes were detected using immunofluorescence.Expression levels of desmin, wtl and nephrin mRNAs in renal tissue were determined using reverse transcription polymerase chain reaction assays.RESULTS: Protein levels in urine and UACR were significantly increased in FSGS model mice compared with Bushenhuoxue-treated and control mice.Body mass and ALB levels were decreased in FSGS mice compared with control and Bushenhuoxue-treated mice.Expression of the wtl protein was observed in control mice.Compared with controls, wtl expression levels were reduced in Bushenhuoxue-treated mice, and to a greater extent in FSGS mice.Nephrin protein expression was widespread in FSGS mice, and significantly reduced in control and Bushenhuoxue mice.Expression levels of wtl and nephrin mRNAs in FSGS mice were lower compared with those in control and Bushenhuoxue-treated mice.Desmin mRNA levels in FSGS mice were reduced compared with those in control and Bushenhuoxue-treated mice.CONCLUSIONS: Bushenhuoxue ameliorated albuminuria in FSGS mice; this was possibly related to the up-regulation of wtl and nephrin, and down-regulation of desmin.
Bushenhuoxue Adriamycin Focal segmental glomerulosclerosis Podocytes wt1 Nephrin Desmin
Chunxia Zuo Xiaoyue Tan Shengqin Jia Mianzhi Zhang Daning Zhang
Nephrology department,Tianjin Police Hospital Department of Pathology,School of Medicine,Nankai University,Tianjin Nephrology department,Tianjin Academy of Traditional Chinese Medicine
国内会议
2014年中华中医药学会补肾活血法分会暨天津市中西医结合学会肾脏病分会学术会
天津
英文
231-242
2014-12-07（万方平台首次上网日期，不代表论文的发表时间）