An electrochemical biosensor for assessment of the antioxidant capacity of antioxidants
An electrochemical biosensor for assessment of the antioxidant activity of antioxidants was fabricated by immobilizing guanine,poly(dimethyldiallylammonium chloride) (PDDA),Fe@Fe2O3 core–shell nanonecklace and glucose oxidase (GOX) in sequence on an activated glassy carbon electrode (GCE).The glucose oxidase (GOX) was kept good bioactivity in the composite membrane.In glucose solution,the hydroxyl radical (·OH) was generated in the membrane via a series of reactions and damaged guanine in situ.As adding antioxidants ascorbic acid,caffeic acid,coumaric acid and resveratrol into the damage system,these antioxidants could alleviate the guanine damage degree.By recording the square wave voltammetric signal change of the guanine,the antioxidant capacities of the four antioxidants were evaluated.The sensor exhibited good reproducibility and stability for rapid assessment of the antioxidant capacity.
Guanine glucose oxidase antioxidants biosensor antioxidant capacity
Rong Chen Huanhuan Xu Li Sun Zhe Liu Xueliang Wang Zhangyu Yu
College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu 273165, China Chemistry and Chemical Engineering, Heze University, Heze 274015, China College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu 273165, China;Chemistry
国内会议
桂林
英文
1-2
2014-04-01（万方平台首次上网日期，不代表论文的发表时间）