Biosensing with Novel Fluorescent Agents
Enzymes bearing environment-sensitive fluorophores can function as biosensors by giving a change in fluorescent signal upon binding to the enzyme substrates.The success of such biosensors (sensitivity,selectivity,etc.)depends on a number of parameters including the location of fluorophore,nature of fluorophore label,and mutation of amino acid residues on the enzymes.When the environment-sensitive fluorophore is placed at an appropriate location on t he enzyme,the change in fluorescent signal can be correlated to the binding of substrate to the enzyme to give the enzyme-substrate (ES) complex,and the subsequent release of substrate from the enzyme active site.In this presentation,an account on how fluorophore-labelled enzyme can report the enzyme activity will be given.
Kwok-Yin Wong
Department of Applied Biology and Chemical Technology and the State Key Laboratory of Chirosciences, The Hong Kong Polytechnic University, Hunghom, Kowloon, Hong Kong SAR, China
国内会议
成都
英文
1-1
2014-11-07（万方平台首次上网日期，不代表论文的发表时间）