A microfluidic Platform for Multiparameter Single-Cell Signaling Measuremets of Tumor Samples
Future personalized medicine in cancer therapy relies on the development of molecular diagnosis.Molecular diagnosis provides more complete and precise pictures of cancer mutations that can guide drug selection and monitor therapeutic outcome.Current technologies for quantitation of the cancer proteome are either qualitative (e.g.,immunohistochemistry) or require large sample sizes (e.g.,flow cytometry).Here,we report a microfluidic platformmicrofluidic image cytometry (MIC)-capable of quantitative,single-cell proteomic analysis of multiple signaling molecules with as little as a few hundred cells.We first tested the microfluidics platform by simultaneous measurement of four critical signaling proteins (EGFR,PTEN,phospho-Akt,and phospho-S6)within the oncogenic PI3K/Akt/mTORsignaling pathway.
Jing Sun Michael Masterman-Smith Mingfei Lang Hsian-Rong Tseng
School of Environmental and Chmical Engeineering, Dalian University, Dalian 116622, China Department of Molecular and Medical Pharmocology, David Geffen School of Medicine University of Cali Medical School, Dalian University, Dalian 116622, China
国内会议
成都
英文
1-2
2014-11-07（万方平台首次上网日期，不代表论文的发表时间）