Highly Sensitive Bioluminescent Detection of MicroRNA based on Hybridization Chain Reaction
MicroRNAs (miRNAs) play important roles in a wide range of biological processes,and their aberrant expressions are associated with various diseases.Here,we develop a label-free sensor for sensitive detection of microRNA on the basis of bioluminescence monitoring the released AMP from the target-triggered hybridization chain reaction-mediated ligation.The introduction of hybridization chain reaction (HCR) not only improves the sensitivity of microRNA assay,but also facilitates the sensitive detection of microRNA by designing specific hairpin probes,Moreover,this bioluminescence assay employs the target-dependent ATP as the reporter without the requirement for complicated immobilization,and separation steps.This highly sensitive and specific assay has potential to become a promising miRNA quantification method in biomedical research and clinical diagnosis.
Hybridization Chain Reaction MicroRNAs Bioluminescence label-free
Jiao Du Guofan Wu Jing Liu Jingxuan Zhang Xudong Guo Xingming Ning Xiaoquan Lu
Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, 730070, China
国内会议
成都
英文
1-2
2014-11-07（万方平台首次上网日期，不代表论文的发表时间）