Polymers from palm oil: biosynthesis and polymerization of a series of ω-hydroxyl fatty acids
(0) Palm oil was used as a substrate to convert fatty acids to ω-hydroxyl fatty acids (ω-HOFAs) by engineered Candida tropicalis yeast strains.The strain (DP522)harboring cytochrome P450 CYP51A13 gives the highest efficiency for the biosynthesis of ω-hydroxyl hexadecanoic acid (ω-HOC16:0) as well as ω-hydroxyl octadecanoic acid(ω-HOC18:1) using pure palmitic acid and oleic acid as substrates, the maximum volumetric yields are 10.5g/L and 25g/L with the ratio of ω-HOFAs to diacids 2.8 and3.9 respectively.When using palm oil (consists five fatty acids) as substrate, by using optimized fermentation conditions (continuous feeding of lipase accelerating hydrolysis of palm oil triglyceride to release free fatty acids), we are able to obtain maximum volumetric yields of 13.8g/L and ll.6g/L for ω-HOC16:0 and ω-HOC18:1 respectively,with ratio of ω-HOFAs to diacids of 7.4 and 1.9.Polyesters, homopolymers as well as copolymers, were successfully synthesized using ω-HOFAs monomers obtained from biosynthesis.The molecular weights (Mw) are 180K for P(ω-HOC16) and 230K for P(ω-HOC 18).The melting point of the polyesters increased from 97℃ to 102℃ when the chain length increases from 14 to 18.The resulting polyesters were found to own advanced tensile testing mechanical properties with Young”s modulus of ca.400MPa and elongation at break of 500%.
palm oil biosynthesis polyester ω-hydroxyl fatty acid poly(ω-hydroxyl fatty acid)
Fei Liu Wenhua Lu Jiali Cai Wenchun Xie Richard Gross Jin Zhu
NSF I/UCRC for Biocatalysis and Bioprocessing of Macromolecules, Department of Chemical and Biologic NSF I/UCRC for Biocatalysis and Bioprocessing of Macromolecules, Department of Chemical and Biologic Ningbo Key Laboratory of Polymer Materials, Ningbo Institute of Materials Technology and Engineering
国内会议
宁波
英文
793-816
2014-11-11（万方平台首次上网日期，不代表论文的发表时间）