Stability enhanced polyelectrolyte-coated gold nanorod-photosensitizer complexes for high/low power density photodynamic therapy
Photodynamic therapy (PDT) is a promising treatment modality for cancer and other malignant diseases, however safety and efficacy improvements are required before it reaches its full potential and wider clinical use.Herein, we investigated a highly efficient and safe photodynamic therapy procedure by developing a high/low power density photodynamic therapy mode (high/low PDT mode) using methoxypoly(ethylene glycol) thiol (mPEG-SH) modified gold nanorod (GNR)-AlPcS4photosensitizer complexes, mPEG-SH conjugated to the surface of simple polyelectrolyte-coated GNRs was verified using Fourier transform infrared spectroscopy;this improved stability, reduced cytotoxicity, and increased the encapsulation and loading efficiency of the nanoparticle dispersions.The GNR-photosensitizer complexes were exposed to the high/low PDT mode (high light dose =80 mW/cm2 for 0.5 min; low light dose =25 mW/cm2 for 1.5 min), and a high PDT efficacy lead to approximately90% tumor cell killing.Due to synergistic plasmonic photothermal properties of the complexes, the high/low PDT mode demonstrated improved efficacy over using single wavelength continuous laser irradiation.Additionally, no significant loss in viability was observed in cells exposed to free AlPcS4 photosensitizer under the same irradiation conditions.Consequently, free AlPcS4 released from GNRs prior to cellular entry did not contribute to cytotoxicity of normal cells or impose limitations on the use of the high power density laser.This high/low PDT mode may effectively lead to a safer and more efficient photodynamic therapy for superficial tumors.
high/low power density AlPcS4 photosensitizer gold nanorods photodynamic therapy (PDT) near-infrared synergistic therapy
Zhenzhi Shi Wenzhi Ren An Gong Xinmei Zhao Yuehong Zou Eric Michael Bratsolias Brown Xiaoyuan Chen Aiguo Wu
Key Laboratory of Magnetic Materials and Devices, & Division of Functional Materials and Nanodevices Department of Biological Sciences, University ofWisconsin-Whitewater, Whitewater, Wisconsin53190, US Laboratory of Molecular Imaging and Nanomedicine, National Institute of Biomedical Imaging and Bioen
国内会议
宁波
英文
965-998
2014-11-11（万方平台首次上网日期，不代表论文的发表时间）