Multicolor nitrogen-doped carbon dots for live cell imaging
(0) Doping carbon dots with nitrogen atoms considerably enhances their fluorescence properties.However, the mechanism by which the carbon dots are doped is not fully understood.We developed a facile bottom-up hydrothermal carbonization(HTC) process that uses glucose and glycine as precursors for the synthesis of photoluminescent nitrogen-doped carbon dots.The as-prepared nitrogen-doped carbon dots were mono-dispersed spherical particles with a diameter of~2.8 nm.The doped nitrogen atoms assumed pyridinic type and pyrrolic type configurations to participate in the nanocrystal structure of the carbon dots.It appeared that the nitrogen doping introduces a new internal structure.The aqueous solution of nitrogen-doped carbon dots showed excitation wavelength-dependent multicolor photoluminescence.Further, these nitrogen-doped carbon dots readily entered the cytoplasm of A549 cancer cells and showed no significant cytotoxicity.The internalized nitrogen-doped carbon dots were localized to the cell membrane and cytoplasm, particularly around the nucleus.Further,the as-prepared, biocompatible, nitrogen-doped carbon dots demonstrated the potential to be used as fluorescent probes for multicolor live cell labeling, tracking, and imaging.
Carbon dots Nitrogen-doped Multicolor Bioimaging
Fengyi Du Sheng Xia Dongqing Wang Shiming Yang Wenrong Xu Aihua Gong Qixiang Shao Jianan Li Ye Hua Miaomiao Zhang Zhou Zhou Jing Yuan Jun Wang Wanxin Peng Li Zhang
Institute of Oncology, Affiliated Hospital of Jiangsu University, Jiangsu University, Zhenjiang,2120 School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang, 212013, P.R.China Institute of Oncology, Affiliated Hospital of Jiangsu University, Jiangsu University, Zhenjiang,2120 Department of Otolaryngology Head and Neck Surgery, Chinese PLA General Hospital, Beijing,100853, P.
国内会议
宁波
英文
1231-1249
2014-11-11（万方平台首次上网日期，不代表论文的发表时间）