The lysis of bacterial cell in oscillating electric field

摘要：

　　A new disruption method using oscillating electric field has been developed in this study.In a low voltage oscillating electric field,Eschericia coli cells were effectively disrupted and recombinant proteins were released showing enzymatic activity.Different oscillating frequencies were tested to optimize the disruption of cells but no significant influence of frequency on cell lysis was observed.This method can be further developed for sterilization of beverages and diary products and used by microfluidic or high throughput screening devices to detect bio-molecules.

关键词： oscillating electric field cell disruption sterilization microfluidic device high throughput screening

作者: Jianfeng Jin Jianmin Qin

作者单位: Department of Biotechnology,Delft University of Technology,Julianalaan 136,2628 BL Delft,the Netherl Key Lab of Advanced Transducers and Intelligent Control System,Ministry of Education,Taiyuan Univers

会议类型: 国内会议

会议名称: 中国电子学会电路与系统学会第二十五届年会

会议地点: 成都

会议语种:英文

页码: 1-13

在线出版日期: 2014-10-01（万方平台首次上网日期，不代表论文的发表时间）