Ratio of γ-H2AX level in lymphocytes to that in granulocytes detected using flow cytometry as a potential biodosimeter for radiationexposure
Purpose:To assess the use of RL/G of γ-H2AX (ratio of γ-H2AX in lymphocytes to that in granulocytes ) in blood as a rapid method for population triage and doses estimation in events of large-scale radiation emergencies.Materials and methods:Blood samples from healthy volunteers exposed to 0-10Gy of 60Co irradiation and cultured for 0-24h were analysed using flow cytometry assay to measure γ-H2AX levels in lymphocytes and granulocytes.The basal levels of RL/G of γ-H2AX in health human blood,the response of RL/G of γ-H2AX to ionizing radiation and its relationship to doses,time intervals after exposure,and individual differences were analyzed in our study.
RL/G of γ-H2AX radiation lymphocytes granulocytes flow cytometry
Zhidong Wang Ying Chen Hailiang Hu Ming Hu Xueqing Zhang Qi Wang Yulei Qiao Haixiang Liu Liping Shen Pingkun Zhou
Department of Radiation Toxicology and Oncology,Beijing Institute of Radiation Medicine,27 Taiping Road,Beijing,100850,China
国内会议
苏州
英文
97-98
2014-05-11（万方平台首次上网日期，不代表论文的发表时间）