High-yield Production of a Chitinase from Aeromonas veronii B565 as a Potential Feed Supplement for Warm-water aquaculture

摘要：

Chitin,present in crustacean shells,insects,and fungi,is the second most plentiful natural organic fiber after wood.To effectively use chitin in a cost-saving and environmentally friendly way in aquaculture,crustacean shells (e.g.,shrimp-shell meal) are supplemented into aquafeed after degradation by chemical methods.Herein,we describe a chitinase from Aeromonas veronii B565,designated ChiB565,,which potently degrades shrimp-shell chitin and resists proteolysis.,We isolated recombinant ChiB565 of the expected molecular,mass in large yield from Pichia pastoris.ChiB565 is optimally,active at pH 5.0 and 50 °C and stable between pH 4.5 and,9.0 at 50 °C and below.

关键词： Chitinase Aeromonas sp . Overexpression Feed supplements Aquaculture

作者: Yuting Zhang Einar Ring Zhigang Zhou Yuchun Liu Yanan Cao Suxu He Fengmin Huo Chubin Qin Bin Yao

会议类型: 国内会议

会议名称: 中国生物工程学会2014年学术年会暨全国生物技术大会

会议地点: 温州

会议语种:英文

页码: 1-1

在线出版日期: 2014-11-07（万方平台首次上网日期，不代表论文的发表时间）