Evaluation of Current Methodologies for Rapid Identification of Methicillin-resistant Staphylococcus aureus Strains
The identification of Staphylococcus aureus strain directly is clinically relevant,but it requires a assay that is both rapid and reliable.Previously,biochemical,immunological,tube coagulase,and agar culture-medium methods have shown variable sensitivity and specificity.However,such methods can not be applied for the direct detection of MRSA from nonsterile specimens such as nasal samples without the previous isolation,capture,or enrichment of MRSA because these samples often contain both coagulas-negative staphylococci (CoNS) and S.aureus,either of which can carry mecA.In this study,we describe a real-time multiplex PCR assay,orfX-PCR,Vitek-2.API-Staph & Vitek-2.These allow the detection of MRSA directly from clinical specimens containing a mixture of staphylococci.MRSA strains can be identified specifically the S.aureus chromosomal orfX gene sequences located to the right of the SCCmec integration site.Methicillin-susceptible S.aureus isolates were correctly reported as susceptible by all methods.In conclusion,these molecular methods and the Vitek2automated system are highly accurate methods for methicillin resistance detection.The multiplex PCR and orfX-PCR method was very suitable for detection of MRSA,the VITEK 2 oxacillin MIC determination proved to be a highly sensitive,rapid and cheap procedure at least equivalent to other phenotypic techniques.
MRSA Identification API-Staph Vitek-2 Multiplex PCR OrfX-PCR
LIU Xiao-chen DENG Yang MIAO Jian CHENG Xian-bin LIU Qun SU Jian-yu XU Zhen-bo Mark E.Shirtliff
College of Light Industry and Food Sciences,South China University of Technology,Guangzhou 510640,Ch Guangzhou Women and Children”s Medical Center,Guangzhou 510623,China;Department of Microbial Pathoge First Affiliated Hospital of Jinan University,Guangzhou 510620,China
国内会议
广州
英文
540-545
2015-07-03（万方平台首次上网日期，不代表论文的发表时间）