Yangjing Capsule Extract Promotes Proliferation of GC-1 Spg Cells via Up-regulated POU3F1 Pathway
(0) Background: As similar to glial cell line-derived neurotrophic factor (GDNF),the Yangjing Capsule (YC) extract could also lead to proliferation of spermatogonial stem cells (SSCs) by motivating phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT) pathway; however,the regulatory effect of YC extract on the expression of POU3F1 still remains unknown.Objective: To determine whether the transcription factor POU3F1 is up-regulated by YC extract through PI3K/AKT signaling pathway to regulate SSCs survival and proliferation.Methods: The cultured GC-1 spermatogonial (spg) cells were treated with 0.01,0.1,and 1 mg/mL YC extract for 48 h.The cell viability was analyzed using MTT assay,while POU3F1 expression was quantitatively detected using real-time polyrnerase chain reaction and Western blot analysis.POU3F 1,GDNF family receptor alpha 1 (GFRα1) short interfering ribonucleic acid (siRNA),and LY294002 (PI3K inhibitor) were applied as blockers to explore the underlying pathway.Results: After 48 h treatment with YC extract,the GC-1 spg cells were proliferated and the POU3F1 expression was significantly increased in a dose-dependent manner.The POU3F1 siRNA partially blocked those effects of YC extract.Both GFRα1 siRNA and LY294002,as upstream blockers,reduced the POU3F 1 expression induced by YC extract.Conclusion: The YC extract promotes proliferation ofGC-1 spg cells via up-regulation of POU3F1.The potential mechanism is that YC extract triggers the activation of PI3K/AKT pathway and then up-regulates POU3F1 expression.
Yangjing Capsule (YC) glial cell line-derived neurotrophic factor (GDNF) spermatogonial stem cells (SSCs) POU3F1
Bin Cai Baofang Jin Xindong Zhang Yuegui Cui Dalin Sun Bin Chen Guanghui Chen Yong Wang Chao Gao
Andrology Department of Integrative Medicine,Zhongda Hospital,Southeast University,Nanjing 210009,Ch ReproductiveMedicineCenter,the Affiliated Drum Tower Hospital,Medical College,NanjingUniversity,Nanj State Key Laboratory of Reproductive Medicine,Clinical Center of Reproductive Medicine,First Affilia Department of Traditional Chinese Medicine,People”s Hospital of Suzhou Gaoxin District,Suzhou 215011
国内会议
长沙
英文
125-136
2015-10-01（万方平台首次上网日期，不代表论文的发表时间）