Comparative pharmacokinetic study of six alkaloids in rat serum after oral administration of fuzi (lateral root of Aconitum carmichaeli Debx)extract in normal and adjuvant arthritis model rats by ultra performance liquid chromatography-mass spectrometry
A simple and sensitive ultra performance liquid chromatography-mass spectrometric (UPLC-MS) method has been developed and validated for simultaneous determination of six alkaloids ofaconitine (AC),hypaconitine (HA), mesaconitine(MA), benzoylaeonine (BAC), benzoylmesaconine (BMA) and benzoylhypaconine (BHA) in normal and adjuvant arthritis (AA) rat serum after oral administration of fuzi (lateral root of Aconitum carmichaeli Debx) extract.The analytes were separated on an ACQUITY UPLC BEH-C18 column within a total running time of 22 min with a mobile phase of 0.1% formic acid-methanol using a gradient program.The calibration curves for all alkaloids showed good linearity in the measured range with correlation coefficient (r) higher than 0.9920.The specificity, linearity, extraction recovery,stability and intra-and inter-day precision were acceptable for biological products.The method was successfully applied to the comparative pharmacokinetics study of the alkaloids in serum of normal and adjuvant arthritis rats after oral administration of fuzi extract.
UPLC-MS Pharmacokinetics Raw fuzi Alkaloids Normal and AA rats
Xuan Wang Lin Zhang Jing Xing Huijin Yang Hua Yin Jianhua Zhang
School of Pharmacy, Zhejiang Chinese Medical University, Hangzhou, China Zhejiang Chinese Medical University First Affiliated Hospital,Hangzhou,China
国内会议
兰州
英文
781-794
2015-08-13（万方平台首次上网日期，不代表论文的发表时间）