Characterization of recombinant anti-CD20 monoclonal antibody expressed in milk of transgenic cattle

摘要：

Rituximab, a mouse/human chimeric anti-CD20 monoclonal antibody (mAb), is the most important mAb of clinical value in patients with non-Hodgkin”s lymphoma and other malignant diseases.However, the commercialization of Rituximab is currently produced by mammalian cell culture, which faces a shortage of production capacity and scale-up production will be inevitably associated with extremely high costs.Here, we established the transgenic cattle expressing the chimeric anti-CD20 mAb in milk.The expressed anti-CD20 mAb was correctly assembled in the mammary gland and the cattle were shown to have anti-CD20 expression more than 1 mg/mL, with the highest level being 6.8 mg/mL.

作者: Ran Zhang

作者单位: China Agricultural University

会议类型: 国内会议

会议名称: 全国农业生物化学与分子生物学第十四届学术研讨会

会议地点: 浙江温州

会议语种:英文

页码: 68-68

在线出版日期: 2015-11-01（万方平台首次上网日期，不代表论文的发表时间）