Effects of different stating on global DNA methylation of aorta in rats with hyperhomocysteinemia
Background The aim of the study is to investigate the effects of different stating on global DNA methylation of aorta in rats with hyperhomocysteinemia.Methods Forty-eight healthy 6-week-old wistar male rats, weighing (160-±10) g, after being fed adaptable for a week,were randomly divided into control group (CON; n=12), hyperhomocysteinemia group (HHcy; n=12) atorvastatin treatment group (AT; n=12) and rosuvastatin treatment group (RT; n=12).After be maintained for 24 weeks, the homocysteine and lipid concentrations in the plasma was measured with the IMX assays.The thoracic aorta was harvested for morphology (HE staining).Extraction aorta tissue global DNA and nuclear extracts, then measure the DNA methyltransferase activity and global DNA methylation levels.
LIU Jing JIA Shao-bin CHEN Shu-lan ZHANG Zheng-jun HOU Jian-jun
Ningxia Medical University Graduate School Department of Cardiology,Heart Center,Affiliated Hospital of Ningxia Medical University
国内会议
北京
英文
257-257
2013-03-20（万方平台首次上网日期，不代表论文的发表时间）