Atorvastatin reduces apoptosis of human coronary artery endothelial cells induced by advanced glycation end products through PI3K/AKT/mTOR pathway

摘要：

Background The aim of the study is to study the mechanism of atorvastatin reducing advanced glycation end products(AGE) induced apoptosis of human coronary artery endothelial cells (HCAEs).Methods There were four groups, (1) Blank control group; (2) AGE group: HCAEs were incubated with AGE (0.1g/L) for 24 hours; (3) AGE+atorvastatin group: HCAEs were incubated with concentration of atorvastatin (1 μmol/L) for1 hour, then incubated with AGE (0.1 g/L) for 24 hours; (4) PI3K inhibitor (LY294002) group: HCAEs were incubated with LY294002 (5000 nmol/L) for 1 hour, then incubated with atorvastatin (1 μmol/L) and AGE (0.1 g/L) for 24 hours.Then, HAECs was stained by ANNEXINV/PI and ANNEXINV/CD144 endothelial microparticles were analyzed by flow cytometry; Western blotting was performed to detect PI3K, p-AKT, p-mTOR protein.

作者: LI Ying LI Cui LI Ji-ming PANG Xiu-feng WANG Jie ZHANG Yi-bo LAI Yah YAO Yi-an LIU Xue-bo

作者单位: Department of Cardiology,Shanghai East Hospital,Tongji University School of Medicine,Shanghai 200120,China

会议类型: 国内会议

会议名称: 第11届中国介入心脏病学大会

会议地点: 北京

会议语种:英文

页码: 267-267

在线出版日期: 2013-03-20（万方平台首次上网日期，不代表论文的发表时间）