SPAR markers revealed genetic diversity of wild populations of Salvia miltiorrhiza Bunge.,an important Traditional Chinese Medicine
Genetic variability in the wild genotypes of Salvia miltiorrhiza Bunge.collected from different parts of China, was analyzed using two remarkable single primer amplification reactions(SPAR) markers, viz., Inter-simple Sequence Repeats (ISSR) and Start Codon Targeted (SCoT).A total of seventy-two individuals comprising of nine wild populations were applied to investigate the existing natural genetic diversity.Four hundred and thirty three (458) amplification fragments were scored by thirty-three (33) SPAR primers, which collectively showed a high polymorphism of 86.90% with a mean polymorphie fragments of 13.88.Meanwhile, their level of diversity at inter-and intra-population levels was significant, with the percentage of polymorphic loci (PPB)ranging from 33.03% to 45.27%.Nei”s gene diversity values (h) varied from 0.124 to 0.167 and Shannon”s diversity values (I) ranged from 0.183 to 0.247 with a genetic diversity at inter-population level 0.508 and genetic diversity at intra-population level 0.140.The estimate gene flow was 0.483.AMOVA analysis of pairwise distances indicated both high variation at intra-population level (46.26%) and inter-population level (53.74%).Five clusters were detected by cluster analysis using the unweight pair-group method with arithmetic average (UPGMA) and STRUCTURE.This study demonstrated that the efficiency of SPAR methods to assess the genetic diversity of wild Salvia miltiorrhiza populations and can be as strating point for future research on the population and evolutionary genetics of this species.
S.miltiorrhiza ISSR SCoT Genetic diversity
Liang Peng Xiao Liang Mei Ru Bo Li Zhengqing Bai Zongsuo Liang Xijun Yan Fenghua Liu
College of Life Sciences, Northwest A & F University, Yangling 712100, China; College of Pharmacy, S College of Life Sciences, Northwest A & F University, Yangling 712100, China College of Life Sciences, Northwest A & F University, Yangling 712100, China;College of Life Science Tianjin Tasly Holding Group Corporation, Tianjin 300000, China
国内会议
武汉
英文
192-202
2015-10-01（万方平台首次上网日期，不代表论文的发表时间）