Simultaneous determination of isorhynchophylline and its metabolites in rat plasma by ultra performance liquid chromatography/quadrupole time-of-flight mass spectrometry
Isorhynchophylline (IR) is one of the major alkaloids from the Uncaria rhynchophylla possessing the effects of lowering blood pressure, vasodilatation, and protection against ischemia-induced neuronal damage.We developed a selective and accurate a UHPLC/Q-TOFMS method for the simultaneous characterization of the main metabolites of IR, and simultaneous quantification of the four metabolites, including 5-oxoisorhynchophyllic acid (5-O-IRA),rhynchophylline (R), 5-oxoisorhynchophyllic acid-22-O-glucuronide (22G-5-O-IRA), and17-O-demethyl-16, 17-dihydro-5-oxoisorhynchophylline (17-O-de-16,17-di-5-O-IR), as well as IR in rat plasma after oral administration of IR at a dose of 40 mg/kg.The pharmacokinetic study showed that IR and its four metabolites were rapidly absorbed with theirAUC0-1 valuesranged from 23.5 to 2.66 μmol·h/L and no enterohepatic recirculation, and 5-O-IRA was a major metabolites in rat plasma, but not IR itself, suggesting that hydrolysis at 22-carboxylic methyl ester and oxidation at C-5 play an important role in in vivo metabolismof IR.Our study provides a comprehensive understanding forthe fate of IR and Uncaria rhynchophylla extracts.
Wen Qi Lizhu Zhao Xudong Yu Dan Yuan
Department of Traditional Chinese Medicine, Shenyang Pharmaceutical University,103 Wenhua Road, Shenyang 110016, China
国内会议
武汉
英文
318-324
2015-10-01（万方平台首次上网日期，不代表论文的发表时间）