Identification of a high-affinity monoclonal antibody against ochratoxin A and its application in ELISA
Ochratoxin A (OTA) is one of the most commonly occurring mycotoxins produced by some species of Aspergillus and can contaminate cereal and cereal products.A high-affinity anti-OTA monoclonal antibody (mAb) was generated from a hybridoma cell line 2D8 using splenocytes from a BALB/c mouse immunized with synthesized OTA-bovine serum albumin conjugate.The mAb 2D8 is specific with high affinity (3.75 × 109L/M).An indirect competitive ELISA (ic-ELISA) was then developed using this mAb for quantitative determination of OTA in corn and feed samples.Using the optimized conditions, there was good linearity between OTA concentration and competitive inhibition (y=-0.6076x+0.2441, R2=0.9923) with the working range from 2.4-23.6μg/kg, IC50 at 7.6μg/kg and lower limit of detection at 1.4μg/kg.The recovery rates in spiked samples were 91.2-110.3%.Of the 56 corn and feed samples, this ic-ELISA and a commercial kit both found the same 13 samples positive for OTA with good linear correlation between the two methods in OTA quantification (R2=0.9706).We conclude that this ic-ELISA can be used for rapid and quantitative screening of corn and feed samples for the presence of OTA.
Xian Zhang Mengjiao Sun Yue Kang Hui Xie Xin Wang Houhui Song Xiaoliang Li Weihuan Fang
Zhejiang University Institute of Preventive Veterinary Medicine, and Zhejiang Provincial Key Laborat College of Animal Science and Technology, Zhejiang A&F University, Lin”an, Zhejiang
国内会议
北京
英文
51-65
2015-11-21（万方平台首次上网日期，不代表论文的发表时间）