Application of Genomic Tools for Enhancing Alfalfa Resistance to Biotic and Abiotic Stresses
Quantitative traits such as biotic and abiotic stress resistance are most likely under the control of multiple genes and interact with environmental factors.Identification of resistance loci that contribute to variation in such complex traits, is a primary challenge in plant breeding and population genetics.In the present study, we used an integrated framework that merges a QTL mapping approach called ”genome-wide association (GWA)” with high-throughput genome sequencing methodology called ”genotyping by sequencing (GBS)” to map disease and drought resistance loci in alfalfa.This framework provides a statistical basis for analyzing marker-trait association using linkage disequilibrium.We have identified a group of significant GBS markers associated with resistance and there are located on different chromosomes.Validation of the markers is in progress.Once validated, these SNP markers can be used for marker-assisted selection.Quantitative traits such as biotic and abiotic stress resistance are most likely under the control of multiple genes and interact with environmental factors.Identification of resistance loci that contribute to variation in such complex traits, is a primary challenge in plant breeding and population genetics.In the present study, we used an integrated framework that merges a QTL mapping approach called ”genome-wide association (GWA)” with high-throughput genome sequencing methodology called ”genotyping by sequencing (GBS)” to map disease and drought resistance loci in alfalfa.This framework provides a statistical basis for analyzing marker-trait association using linkage disequilibrium.We have identified a group of significant GBS markers associated with resistance and there are located on different chromosomes.Validation of the markers is in progress.Once validated, these SNP markers can be used for marker-assisted selection.
Long-Xi Yu
Department of Plant Pathology, Washington State University, USA
国内会议
安徽蚌埠
英文
345-347
2015-10-25（万方平台首次上网日期，不代表论文的发表时间）