Serum soluble major histocompatibility complex class Ⅰ-related chain A/B expression in patients with alcoholic liver disease in Hainan Li community
Background/Aims To study theexpression and clinical significance of serum soluble major histocompatibility complex class Ⅰ-related chain A/B (sMICA/B), and its correlation with percentage of CD4+, CD8+, and NK cells, Liver fibrosis screening test, and liver enzymes in alcoholic liver disease (ALD).Methods Hainan Li ALD patients (n =141) and healthy Li subjects (n =100) were enrolled for the study.Liver enzymes were measured using automatic biochemical analyzer and Liver fibrosis screening test was used to study the correlation.In addition, sMICA/B expression in serum and percentage of CD4+, CD8+, and NK cells were determined using ELISA and flow cytometry respectively.Results Liver fibrosis screening test results and liver enzymes concentration were significantly higher (bothp<0.01), whereas the expression of sMICA and sMICB was significantly indifferent (p>0.01) between ALD patients and healthy controls.However, percentage of CD4+, CD8+, and NK cells were statistically lower in ALD patients than in healthy controls.The Kendall”s tau-b correlation coefficientfor sMICA and sMICB/sMICA and LV was 0.561 and 0.120 respectively (p<0.01).Pearson correlation coefficient of sMICA with the percentage of CD4+, CD8+%, and NK cells was-0.587,-0.525, and-0.232 respectively, whereas the coefficient of sMICB was-0.590,-0.554, and-0.292 respectively (p<0.01).Conclusion 1.Liver fibrosis screening test is an excellent non-invasive approach for the diagnosis of hepatic fibrosis and shows significant correlation with liver enzymes.2.sMICA and sMICB failed to assess the degree of hepatic fibrosis.3.Decreased percentage of CD4+, CD8+, and NK cells were attributed as one of the risk factors for ALD.
Hainan Li population alcoholic liver disease soluble major histocompatibility class Ⅰ-related chain A/B natural killer cells
Xiaobin Wei BiQiong Ren Danqin Lin Bin Luo Xianxian Fu Chunyun Li Huan Xia Xi Xiao Ping Yu
Department of Immunology, College of Basic Medical Sciences, Central South University, 88 Xiangya Ro Department of Immunology, College of Basic Medical Sciences, Central South University, 88 Xiangya Ro Clinical laboratory, The Second people”s Hospital of Hainan Province, 24 Aimin Road ,Wuzhishan 57220 Clinical laboratory, Affiliated Haikou Hospital of Xiangya Medical College in Central South Universi
国内会议
第九届粤桂琼检验医学学术会议暨海南省医学会检验医学专业委员会2015年学术年会
海南陵水
英文
1-12
2015-10-01（万方平台首次上网日期，不代表论文的发表时间）