Quantification of anti-sperm antibody and soluble MICA/MICB levels in the serum of infertile people of the Li nationality in China
Objective: To investigate the presence of anti-sperm antibodies (AsAb) and the correlation between AsAb positivity and the expression of soluble major histocompatibility complex class Ⅰ chain-related A and B (sMICA or sMICB) in the sera of infertile people of the Li nationality from Hainan, China.Method: A total of 136 people (68 couples) from five villages in the Wuzhishan region, Hainan province participated in this study.Among them, 31 couples were included in the fertile group and 37 couples in the infertile group.AsAb and sMICA/sMICB levels in serum were detected by ELISA.The median sMICA/sMICB levels between and among groups were compared by Mann-Whitney rank U testing and Kruskal-Wallis H testing, and the AsAb positivity rate was compared by Pearson Chi-Square testing.Correlation analysis was performed by calculating the Spearman”s rho coefficient for nonparametric data.Results: The serum levels for the fertile group (AsAb: 15.5 ”4.0~127.0” U/ml, sMICA: 18.33 ”13.30~52.40” pg/ml, sMICB: 27.72 ”18.63~47.43” pg/ml) were not statistically different from those for the infertile group (AsAb: 18.0 ”9.8~95.0” U/ml, sMICA: 20.95 ”15.78~23.81” pg/ml, sMICB: 26.26 ”18.06~61.38” pg/ml).However, grouping based on AsAb positivity revealed a statistically significant difference for the sMICA/sMICB levels (AsAb positive group: sMICA: 5.56 ”4.30~17.23” pg/ml, sMICB: 16.13 ”7.54~25.43”pg/ml; AsAb negative group: sMICA: 22.00 ”18.05~66.13” pg/ml, sMICB: 36.51 ”20.53~67.22” pg/ml;P<0.01).These results suggest that AsAb is negatively associated with both sMICA (Spearman”s coefficient,-0.475, P<0.01) and sMICB (Spearman” s coefficient,-0.381; P<0.01).The analysis also shows that sMICA levels are positively associated with sMICB levels (Spearman”s coefficient, 0.635; P<0.01).Conclusion: AsAb can be detected in the serum of fertile and infertile Li people.however, there appears to be limited clinical value in the conventional detection ofAsAb, sMICA and sMICB in serum for diagnosing infertility.People with positive AsAb expression have lower levels of sMICA/sMICB expression in serum,which may be one mechanism by which people produce AsAb.
The Li nationality anti-sperm antibody infertility sMICA/sMICB
Xiaobin Wei Ping Yu Zhouxin Han BiQiong Ren Xi Xiao Danqin Lin Bin Luo Xianxian Fu Chunyun Li Huan Xia
Department of Immunology, College of Basic Medical Sciences, Central South University, 88 Xiangya Ro Department of Immunology, College of Basic Medical Sciences, Central South University, 88 Xiangya Ro The Second people”s Hospital of Hainan Province, 24 Aimin Road ,Wuzhishan 572200, China Clinical laboratory, Affiliated Haikou Hospital of Xiangya Medical College in Central South Universi
国内会议
第九届粤桂琼检验医学学术会议暨海南省医学会检验医学专业委员会2015年学术年会
海南陵水
英文
13-21
2015-10-01（万方平台首次上网日期，不代表论文的发表时间）