Overexpression of long non-coding RNA MEG3 inhibits proliferation of hepatocellular carcinoma Huh7 cells via negative modulation of miRNA-664

摘要：

　　Background :Evidence is accumulating that long non-coding RNAs (lncRNAs) are involved in human tumorigenesis and dysregulated in many cancers, including hepatocellular carcinoma (HCC).Maternally-expressed gene 3 (MEG3) is a lncRNA overexpressed in HCC cells that inhibits HCC progression; however, the mechanism through which MEG3 exerts its oncogenic activity remains largely unknown.Methods: To explore the underlying regulation mechanism of MEG3, the interaction between gene and gene was examined using a luciferase reporter assay.Expression levels were selectively altered in HCC cells and by transfection of MEG3 or antisense nucleic acids for miR-664.Relative expression levels were detected by RT-PCR, The proliferation inhibition of cells were detected by CCK-8 assay ,the protein expression levels were detected by Western blot,Promoter Binding TF Profiling Plate array and Chromatin immunoprecipitation (ChIP) assay were used to comfirm the transcript factors binding to promoter region of MEG3.Results:we demonstrated that MEG3 is down-regulated in HCC tissues.MEG3 over-expression imposes another level of post-transcriptional regulation, whereas MEG3 overexpression reduces the expression of the miRNA-664 target gene, ADH4, through competitive ”sponging” miRNA-664.In addition, NF-KB may affect transcription of MEG3 by directly binding to the promoter region.Conclusion:Our data revealed that NF-KB may affect the transcript of MEG3.MEG3 overexpression inhibited the proliferation of HCC cells, at least in part by affecting miRNA-664-mediated regulation of ADH4.Together,these results suggest that MEG3 is a suppressor of tumor which acts in part through ”sponging”miR-664.

关键词： long non-coding RNA MicroRNA-664 maternally expressed gene 3 hepatocellular carcinoma Alcohol dehydrogenase 4

作者: Jin-Hua He Ze-Ping Han Ji-Ming Liu Mao-Xian Zhou Yu Bing Lv Yu-Guang Li Ming-Rong Cao

作者单位: Department of Laboratory, Central Hospital of Panyu District, Guangzhou, Guangdong 511400 Department of General Surgery, First Affiliated Hospital, Jinan University, Guangzhou 510630, China

会议类型: 国内会议

会议名称: 第九届粤桂琼检验医学学术会议暨海南省医学会检验医学专业委员会2015年学术年会

会议地点: 海南陵水

会议语种:英文

页码: 49-58

在线出版日期: 2015-10-01（万方平台首次上网日期，不代表论文的发表时间）