Long noncoding RNARP5-833A20.1inhibitsproliferation and metastasis and cell-cycleby suppressing NFIAexpression in U251 cell
Backgrounds& Aims:Early reports suggest that nuclear factor IA (NFIA) plays important roles in glioma pathogenesis.Our recent study demonstrated that Long noncoding RNA (1ncRNA) RP5-833A20.1 suppressed the expression of NFIA in THP-1 macrophage-derived foam cells.However, the effect and possible mechanism of RP5-833A20.1 on glioma remain unclear and whether NFIA-dependent pathway involving in this progress has not yet been explored.Here, we investigated the mechanisms by which the RP5-833A20.1 regulatesthe expression of NFIA in glioma.Methods:The expression of RP5-833A20.1 and NFIA was determined from U251 cells and clinical samples by RT-qPCR.The effects of RP5-833A20.1 on cell proliferation, invasion, cell cycle, and apoptosis were evaluated by in vitro assays.The potential protein expression changes were investigated by Western-blotting.The methylation status of the CpGisland in NFIA promoter was explored by bisulfite sequencing.Results:We found that RP5-833A20.1 expression was down-regulated, whereas NFIA expression was up-regulated in glioma tissues compared to corresponding adjacent nontumor tissues from 20 glioma patients.Overexpression of RP5-833A20.1 inhibited proliferation and cell-cycle progression, and induced apoptosis in U251 cells.Both mRNA and protein levels of NFIA were markedly inhibited by overexpression of RP5-833A20.1 in U251 cells.Overexpression of RP5-833A20.1 increased the expression of miR-382-5p in U251 cells.BSP assay revealed that over-expressing RP5-833A20.1 enhanced the methylation level of NFIA promoter.Concluions:This study demonstrates that RP5-833A20.1 inhibits tumor cell proliferation, induces apoptosis, and inhibits cell-cycle progression by suppressing NFIA expression in U251 cell.Collectively, these results representRP5-833A20.1as a novel therapeutic target for glioma.
LncRNARP5-833A20.1 NFIA Glioma U251 cell methylation
Chun-Min Kang Yan-Wei Hu Ying Nie Jia-Yi Zhao Shu-Fen Li Shuai Chu Hai-Xia Li Qing-Shui Huang Yu-Rong Qiu
Laboratory Medicine Center, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 5105 Department of anesthesiology, Guangdong 999 Brain Hospital, Guangzhon, Guangdong 510510, China
国内会议
第九届粤桂琼检验医学学术会议暨海南省医学会检验医学专业委员会2015年学术年会
海南陵水
英文
72-83
2015-10-01（万方平台首次上网日期，不代表论文的发表时间）