Simultaneous determination of 27 hormones in fish, milk and water by solid-phase extraction and ultra-performance liquid chromatography-tandem mass spectrometry
A rapid analytical procedure for the simultaneous determination of twenty seven hormones in fish, milk and water samples by solid-phase extraction (SPE) and ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) has been developed.These hormones, considered as endocrine-disrupting chemicals (EDCs), contained nineteen endogenous and eight exogenous.The method involves extraction with acetonitrile and purification by a HLB SPE cartridge.In order to optimize purification effect of milk and fish matrixes, a comparative study using a diphasic dialysis and matrix solid-phase dispersion (MSPD), was weak in achieving the maximum retention of analytes and best elimination of matrix components.Using the optimized protocol of a HLB cartridge, limits of quantification (LOQs) were obtained ranging from 0.1 to 49.5 ng g-1 for water samples, from 0.1 to 39.1 ng g-1 for milk samples and from 0.1to 49.5 ng g-1 for zebrafish (Danio rerio) samples.Spiked recoveries were within 69.1-120.5% of nominal concentrations.Performance characteristics permit the use of this methodology for 27 hormones determination in fish, milk, water samples.Hydrocortisone (Hd), androstenedione (AN), stanozolol (STD), progesterone (P4), 17-hydroxyprogesterone (17-OHP) and 17-β-Estradiol (E2) were found in milk ranging from 0.21 to 11.41 ng g-1.P4 and trenbolone (TB) were observed in zebrafish at 5.73 and 7.45 ng g-1, respectively.This multi-residue method was used for quantitation of hormone levels in a model organism, zebrafish, tested in endocrine disruptor screening experiments.Meanwhile, this technology would play an important role in evaluating the presence of target EDCs in food and environmental samples.
Hormones Endocrine-disrupting chemicals Ultra-performance liquid chromatography-tandem mass spectrometry Solid phase extraction
Yang Mei Mang You-ning Gui Wen-jun Zhu Guo-nian Ren Yi-ping
Institute of Pesticide and Environmental Toxicology,Zhejiang University,388 Yuhangtang Road,Hangzhou Zhejiang Provincial Center for Disease Control and Prevention,3399 Binsheng Road,Binjiang District,H
国内会议
第十四届全国农药学科教育科研研讨会暨赵善欢学术思想与研究实践讨论会
广州
英文
151-173
2014-08-03（万方平台首次上网日期，不代表论文的发表时间）