Molecular cloning and characterization of the 5”-regulatory region of the bovine Fatty acid binding protein 4 gene
Fatty acid binding protein 4 (FABP4) is a member of FABP family of proteins that bind fatty acids and play important roles in fatty acid uptake and intracellular transport.In the present study, we cloned the5”-regulatory region of bovine FABP4 and identified its transcription initiation sites.Sequence comparative analysis demonstrated amino acids and promoter sequences of FABP4 were highly conservative in mammals.Real-time PCR analysis revealed the products of bovine FABP4 were very highly expressed in subcutaneous adipose tissue.Serial deletion constructs of the bovine FABP4 5”-regulatory region evaluated in a dual-luciferase reporter assay showed that 209 base pairs upstream from the transcrption initiation site was its core promoter.Electrophoretic mobility shift assay combined with site-directed mutation experiment indicated that peroxisome proliferator activated receptor gamma (PPARγ), CCAAT/enhancer-binding protein beta (C/EBPβ) and sterol regulatory element-binding protein (SREBP) were important transcription factors for bovine FABP4.These results provide an important basis for further understanding the regulation of bovine FABP4.
Cattle CCAAT/enhancer-binding protein beta Electrophoretic mobility shift assay Fatty acid binding protein 4 Peroxisome proliferator activated receptor gamma Sterol regulatory element-binding protein
Anning Li Zhidong Zhao Yaran Zhang Changzhen Fu Mingming Wang Linsen Zan
College of Animal Science and Technology, Northwest A&F University, Yangling,Shaanxi 712100, P.R.Chi College of Animal Science and Technology, Northwest A&F University, Yangling,Shaanxi 712100, P.R.Chi
国内会议
陕西杨凌
英文
39-52
2015-11-16（万方平台首次上网日期，不代表论文的发表时间）