Sequence variants of the LCORL gene and its associationwith growth and carcass traits in Qinchuan cattle in China
Molecular marker-assisted selection is a better way to satisfy the growing customer requirement with the development of beef cattle growth and breeding research.Fore now, QTL (quantitative trait locus) for cattle growth and carcass traits, just like body height, body length and carcass weigh has been detected on bovine chromosome 6.In this study, LCORL (ligand dependent nuclear receptor corepressor-like) was selected as the potential positional candidate gene located in chromosome 6 which is closely connected with the bovine growth and carcass traits.A total of 360 Qinchuan beef cattle were used to detect mutations in exon, exon neighboring region and the promoter region of the bovine LCORL gene.The methods for SNPs detection were PCR-RFLP(PCR Restriction Fragment Length Polymorphism) and CRS-PCR (Created Restriction Site PCR), and the results of this study shows: there were two variations in intron regions, the other four variations were located in the promoter region.Linkage disequilibrium analysis and haplotype analysis indicated that L78-Q4 was strong linkage disequilibrium, A T G C G C (16.2%) and G C G C A T (16.7%) had higher haplotype frequencies, G C A C A C (0.8%) and G T A C A T (0.7%) had lower haplotype frequencies.Correlation analysis indicated that SNP g.INT+52098A>G was significantly associated with slaughter weight and carcass weight.Based on the research, the LCORL can be choiced as a candidate gene that can contribute to improved marker-assisted selection for the meat performance of Qinchuan beef cattle.
LCORL Qinchuan beef cattle Polymorphism PCR-RFLP CRS-PCR Association study
Yujiao Han Yu Chen Yu Liu Zhixiong Li Si Chen Wei Liang Xiaolin Liu
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China
国内会议
陕西杨凌
英文
63-68
2015-11-16（万方平台首次上网日期，不代表论文的发表时间）