Global DNA methylation was changed by a maternal high lipid and high energy diet during gestation and lactation in male adult mice liver
Epigenetic mechanism has been suggested to explain the effects of the maternal diet on the development of disease in offspring.The present study aimed to observe the effects of a maternal high lipid and high energy diet (HLE) on the DNA methylation pattern of male offspring in mice.Female C57BL/6j mice were fed with HLE diet during gestation and lactation.The genomic DNA methylations at promoter sites of genes in liver, mRNA and protein levels of selected genes related to lipid and glucose metabolism were measured by micro-array, real time-PCR and western blot.The results indicated that the percent of methylated DNA in offspring from dams fed with HLE diet was significantly higher than that from chow diet and most of these genes were hypermethylated on promoter regions.The nuclear protein content and mRNA levels of hypermethylated genes, such as PPARγ and LXRα, were decreased significantly in HLE group in offspring.The results suggested that the DNA methylation profile in adult offspring liver was changed by the maternal HLE diet during gestation and lactation.
Maternal diet Offspring Hypermethylation Epigenetic Mechanism
Huanling Yu Shan Dong Lifang Gao Li Li Yuandi Xi Weiwei Ma Linhong Yuan Rong Xiao
School of Public Health,Beijing Key Laboratory of Environmental Toxicology,Capital Medical Universit School of Public Health,Capital Medical University and Beijing Key Laboratory of Environmental Toxic
国内会议
北京
英文
37-52
2015-06-01（万方平台首次上网日期，不代表论文的发表时间）