Assessment of Haplotype Variation in Bovine AMPD1 Gene for Association with Growth and Carcass Traits in Qinchuan Beef Cattle
The AMPD1 gene plays an important role in the purine nucleotide cycle and energy metabolism in skeletal muscle.In the present study, polymorphism of the AMPD1 gene was detected by PCR-SSCP and DNA sequencing methods in 215 individuals from Qinchuan beef cattle breed.The result of DNA sequence showed two mutations by comparisons with NC_007301.The g.19416T>C and g.19421A>G mutation were detected in intron 11 ofbovine AMPD1 gene.The sequencing results of difference genotypes showed that four kinds of haplotypes, named: A (T-A), B (T-G), C (C-A) and D (C-G); and the five diplotypes were conflated and described as: AA (T-A/T-A), BC (T-G/C-A or C-G/r-A), AC (T-A/C-A), CC (C-A/C-A) and CD (C-A/C-G).Individuals with diplotype BC was significantly associated with carcass weight (P<0.05, n=56).Animals with the new heterozygote genotype had greater carcass weight than those with the other diplotypes.They may be used as a possible candidate for marker-assisted selection in Qinchuan beef cattle breeding program.
AMPD1 gene mutation haplotype growth and carcass traits Qinchuan beef cattle
Xiaolin Liu Hua He Zhang Huilin Yulan Gu Yu Liu
College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, P.R.China
国内会议
陕西杨凌
英文
92-98
2011-05-01（万方平台首次上网日期，不代表论文的发表时间）