The Participation of p38 MAPK Pathway in Calcium Signal-mediated Lipid Accumulation in Primary Preadipocytes of Mice
In the present study we have examined whether p38 mitogen activated protein kinase (p38MAPK) signaling pathway interacts with calcium signaling on lipid accumulation in primary preadipocytes of mice.The primary preadipocytes were treated with p38 MAPK inhibitor SB203580,blockers and excitomotors of calcium channel for 24h respectively.Intracellular triglyceride (TG)content was measured with Triglyceride kit, and lipid accumulation was determined by Oil Red O stainning Meanwhile, the mRNA expression levels of nuclear factor peroxisome proliferators-activated receptor gamma (PPARγ) gene, fatty acid synthetase (FAS) gene, lipoprotein lipase (LPL) gene,vitamin D receptor (VDR) gene, extracellular Ca2+-sensing receptor (CaSR)gene were analyzed with Quantitative PCR method, the protein and phosphorylation levels of p38, MKK6 and MAPKAPK2were tested with Western Blotting.The data showed that Intracellular TG content and the mRNA expression levels of PPARγ, FAS, LPL in N group and the L group as well as FAS, LPL in C group were increased significantly (P<0.01) compared to that of the control.Whereas intracellular TG content and the mRNA expression levels of PPARγ, FAS in B group as well as intracellular TG content and PPARγ, FAS, LPL in SB group and B+SB group were significantly decreased (P<0.01).VDR mRNA expression level was decreased in B and C groups (P<0.01) and SB group.Data of Western blotting showed that p38, MKK6and MAPKAPK2 phosphorylation levels were increased in N and L groups(P<0.0 1)and decreased in SB added groups (P<0.01).These findings suggest that p38 MAPK pathway and calcium signaling regulate lipid accumulation in mice preadipocytes at part through changing PPARγ, FAS and LPL mRNA expression.p38 MAPK pathway participates in calcium signal-mediated lipid accumulation in mice preadipocytes at least partly through regulating VDR mRNA expression.Similarly, calcium signal changes the mRNA expression levels and protein phosphorylation levels of MKK6 and MAPKAPK2.
p38 MAPK pathway calcium signaling primary preadipocytes lipid accumulation
Chao Sun Renli Qi Li Wang Jun Yan Yong Wang
College of Animal Science and Technology, Northwest A&F University,Yangling, Shaanxi 712100, China
国内会议
陕西杨凌
英文
104-111
2011-05-01（万方平台首次上网日期，不代表论文的发表时间）