The Advantages of Low Density Lipoproteins in the Cryopreservation of Bull Semen
Egg low-density lipoprotein (LDL) was added at concentrations (w/v) of 7%, 8% or 9% to the extenders used to freeze bull semen and its effects on seminal parameters and antioxidant activities of frozen-thawed sperm were assessed.Analysis of data showed that sperm exposed to 8% LDL exhibited the greatest percentages of sperm motility, acrosome integrity and membrane integrity,compared to the control which differed from the treatment groups by replacing LDL with 20% egg yolk(P<0.05).No difference was observed for membrane integrity between 8% and 9% LDL groups(P>0.05).The extender supplemented with LDL did not exhibit improvement in SOD levels.However,8% LDL group favored the highest antioxidant activities of CAT, GSH-Px and GSH in comparison to other groups (7%, 9% LDL and the control) (P<0.05).No difference was observed for CAT activity between 9% LDL and the control group.In conclusion, sperm cryopreserved in the extender containing8% LDL in place of egg yolk exhibited the greatest percentages of post-thaw sperm motility, acrosome integrity and membrane integrity, in comparison with the control, and favored the highest antioxidant activities of CAT, GSH-Px and GSH in comparison with other groups.The replacement of egg yolk by LDL in the composition ofextenders was beneficial for bull sperm cryopreservation.
Low-density lipoproteins Cryopreservation Bull semen Semen quality parameters Antioxidant activities
Jian-Hong Hu Zhong-Liang Jiang Rui-Kai Lv Qing-Wang Li Shu-Shan Zhang Lin-Sen Zan Yao-Kun Li Xin Li
College of Animal Science and Technology, Northwest A & F University,Yangling, Shaanxi 712100, P.R.C College of Animal Science and Technology, Northwest A & F University,Yangling, Shaanxi 712100, P.R.C Husbandry and Veterinary Research Institute, Shanghai Academy of Agricultural Sciences,Shanghai 2011
国内会议
陕西杨凌
英文
295-302
2011-05-01（万方平台首次上网日期，不代表论文的发表时间）