Effects of Vitamin E Supplementation in Extender on Frozen-thawed Bovine Semen Preservation
The maturing sperm cells discard the majority of their cytoplasm during the final stages of spermatogenesis and lose some of their defense-enzymes.The purpose of the present study was to investigate the effects of Vitamin E supplementation on standard semen quality parameters and anti-oxidant activities of frozen-thawed bovine sperm.Vitamin E was added at concentrations of 0.5,1.0, 1.5 and 2.0 mg/ml to bovine semen cryoprotective medium.The results showed that the sperm motility and VSL, STR values in the extender supplemented with 1.0 and 1.5 mg/ml Vitamin E were significantly higher than that of other concentrations (p<0.05).The percentages of acrosome-intact and membrane-intact sperm were significantly improved (p<0.05) by supplementing with 1.5 mg/ml Vitamin E.In biochemical assays, the extender supplemented with Vitamin E did not exhibit significant improvement in SOD levels, compared with the control (p>0.05).Compared with the other groups, CAT levels were demonstrated to be greater with the supplementation of Vitamin E at 1.0 and1.5 mg/ml (P<0.05).The extender supplemented with 1.5 mg/ml Vitamin E caused the highest levels of GSH-Px, compared with other groups (p<0.05).The GSH activity was significantly higher with the supplementation of 0.5, 1.0 and 1.5 mg/ml Vitamin E, compared with 2.0 mg/ml Vitamin E group and control (p<0.05).Moreover, increasing the doses of Vitamin E decreased sperm antioxidant activities,the extender supplemented with 2.0 mg/ml Vitamin E caused the lowest levels of GSH-Px and GSH activities, compared with other treatment groups (p<0.05).In conclusion, the beneficial effects of Vitamin E noted in the present study can be attributed to the antioxidant characteristics.Vitamin E supplementation in the extender reduced the lipid peroxidation potential and improved semen quality during the freezing-thawing.More researches are needed to evaluate and understand the precise physiological role of Vitamin E in reproduction.
Bovine semen Vitamin E,Cryopreservation,Standard semen quality parameters,Anti-oxidant activities
Jian-Hong Hu Xian-Lin Zhao Wan-Qiang Tian Lin-Sen Zan Qing-Wang Li
College of Animal Science and Technology, Northwest A & F University, Yangling,Shaanxi 712100, P.R.C Hainan Vocational and Technical Institute of Biological Sciences, Haikou, Hainan 57000, P.R.China Yangling Vocational and Technical College, Yangling, Shaanxi 712100, P.R.China College of Animal Science and Technology, Northwest A & F University, Yangling,Shaanxi 712100, P.R.C
国内会议
陕西杨凌
英文
303-310
2011-05-01（万方平台首次上网日期，不代表论文的发表时间）