Effects of Rhodiola Sachalinensis Saccharide on Bovine Spermatozoa During Freezing-thawing
This study measured the hypothesis that Rhodiola Sachalinensis Saccharide (RSS) had the positive effect on bovine cryopreservation sperm.RSS was extracted from rhodiola sachalinensis (R.sachalinensis) and added into bovine frozen diluents with 2, 3, 4, 5, 6% (w/v), repectively.After freezing-thawing, the percentages of total motile sperm (TMS), rapid progressive motile spermatozoa(RMPS) of bovine frozen-thawed sperm were significantly higher with the concentrations of 4%(48.24%, 48.43%) and 5% (52.66%, 49.64%) RSS than other groups, respectively (P<0.05).The plasma membrane integrity (PMI) and mitochondrial activity (MA) of bovine frozen-thawed sperm were significantly lower at the concentrations of 2%, 3% and 6% RSS than others (P<0.05),respectively.The values of AI in the groups of 2% and 3% RSS was significantly lower than other groups (P <0.05).Compared with the groups of supplementing 4%, 5% and 6% RSS, the values of MDA were higher in the control group and the concentration of 2%, 3% RSS (P<0.05), and there was no difference among the addition of 4%, 5% and 6% RSS (P>0.05).The contents of vitamin E were significantly lower in control group and groups with the concentrations of 2%, 3%, 4% RSS than the groups with 5% and 6% RSS (P<0.05).The results demonstrated that the comet rate (CR) was improved significantly in the groups with supplemented 4% (29.55%) and 5% (25.80%) RSS (P<0.05).In the groups of adding 2% and 3% RSS, the CR, tail DNA (TD) and tail moment (TM) were increased significantly (P<0.05).
Freezing-thawing Rhodiola sachalinensis saccharide Bovine Sperm Cryoprotectant
Zhong-liang Jiang Jian-hong Hu Wen-ye Li Ting Zhang Qing-wang Li
College of Animal Science & Technology, Northwest A & F University,Yangling, ShaanXi province, 71210 College of Animal Science & Technology, Northwest A & F University,Yangling, ShaanXi province, 71210
国内会议
陕西杨凌
英文
311-319
2011-05-01（万方平台首次上网日期，不代表论文的发表时间）