Mixed infection of avian hepatitis E virus and Marek” s disease virus in hens in China
The average mortality of a flock of Hy-Line variety brown hens in Jiangsu Province, China, was 0.44% over 20weeks.Post-mortem inspection revealed that the Pathological changes was consistent with hepatitis-splenomegaly syndrome, including hepatomegaly, serosanguineous peritoneal fluid and hepatic subcapsular haemorrhages.Microscopic lesions of the liver were characterized by multifocal necrotizing hepatitis and mild lymphocytic infiltration.Amounts of avian HEV RNA samples were detected from 56%to 80% of the liver from clinical onset chickens (14/25) and clinical healthy chickens (4/5) by polymerase chain reaction (PCR).Sequencing analysis of a 242-base-pair gene fragment from clinical onset chicken and clinical healthy chicken showed 97.9% to99.2% nucleotide sequence identity with avian HEV genomes, whereas identities ranged from 77.3% to 97.9% compared with other representative sequences.In addition, 40% to 48% of liver samples from clinical healthy chickens (2/5) and clinical onset chickens(12/25) were detectable amounts of MDV meq DNA by PCR, respactively.Sequencing analysis of a 396-base-pair gene fragment showed that the MDV strain belonged to serotype 1 strains.The co-infection rate of avian HEV and MDV was 30% (9/30).In contrast to other reported avian HEV outbreaks that occurred in broiler breeder in China, in the present investigation clinical avian HEV was identified in Hy-Line variety brown hens.This study first found and reported the mixed infection of MDV and avian HEV in chicken.
Avian HEV MDV Co-infection Hy-Line variety brown hens
Shuqing Yang Shuhong Sun
Shuqing Yang; Shah Dong Agricuhural University, 61 daizong Rd, Tai an, Shah dong Province, 271000
国内会议
天津
英文
92-96
2015-11-06（万方平台首次上网日期，不代表论文的发表时间）