Picroside Ⅱ plays neuroprotective effect on cerebral ischemic injury in rats
This paper aimed to explore the protective effects of picroside Ⅱ against the neuronal apoptosis and changes in morphology and structure that follow cerebral ischemic injury in rats.A focal cerebral ischemic model was established by inserting a monofilament thread to achieve middle cerebral artery occlusion (MCA0) in 60 Wistar rats, and intraperitoneal injections of picroside Ⅱ (20 mg/kg) were administered.The neurobehavioral functions were evaluated with the modified neurological severity score (mNSS) test.The cerebral infarct volumes were measured with tetrazolium chloride (TTC) staining.The morphology and ultrastructure of the cortical brain tissues were observed with hematoxylin-eosin staining and transmission electron microscopy, respectively.The apoptotic cells were counted with terminal deoxynucleotidyl transferase dUTP nick-end labeling and flow cytometry, and pERK1/2 expression was determined by immunohistochemical assay.The results indicated that neurological behavioral malfunctions and cerebral infarcts were present in the MCA0 rats.In the model group, the damage to the structures of the neurons and the blood brain barrier (BBB) in the cortex was more severe, and the numbers of apoptotic cells, the early apoptotic ratio (EAR) and pERK1/2 expression were significantly increased in this group compared to the control group (P<0.05).In the treatment group,the neurological behavioral function and the morphology and ultrastructure of the neurons and the BBB were improved, and the cerebral infarct volume, the number of apoptotic cells, the EAR and pERK1/2 expression were significantly decreased compared to the model group (P<0.05).These results suggest that picroside Ⅱ reduced apoptosis and improved the morphology and ultrastructure of the neurons and the BBB and that these effects resulted in the recovery of the neurobehavioral function of rats with cerebral ischemia.
picroside Ⅱ cerebral ischemia apoptosis morphology ultrastructure rats
Yunliang Guo Tingting Wang Li Zhao Meizeng Zhang Haitao Pei
Institute of Cerebrovascular Diseases,Affiliated Hospital of Qingdao University,Qingdao Shandong 266003,P.R.China
国内会议
北京
英文
133-146
2015-04-24（万方平台首次上网日期，不代表论文的发表时间）