Effects of feeding E.adenophorum-containing feedstuffs on the cell cycle, apoptosis and mRNA expression of Bax, Bcl-2,Caspase-3, 8, 9 of kidney in Saanen Goat
The experiment was conducted to explore the effects of feeding Saanen goat E.adenophorum-containing feedstuffs on apoptosis, cell cycle progression, and relative expression quantity of apoptosis genes of Bax, Bcl-2,caspase-3, 8, 9 in kidney through flow cytometry and qRT-PCR.Sixteen Saanen goats randomly divided into four groups were fed on control diet (non-E.adenophorum) and experimental diets mixed with 40%, 60%, 80% E.adenophorum leaves for 3 months respectively.As tested by flow cytometry, the results showed that the percentage of apoptosis in renal cell was increased and the normal renal cells obviously were decreased in experimental groups as compared to control.Renal cells in G0/G1 phase were much higher, and lower in S phase and proliferation index value in experimental groups which means E.adenophorum importantly arrest the cell cycle transition from G0/G1 to S phase which made renal cell can”t complete the cell cycle activity and increased cellular apoptosis in the kidney.qRT-PCR results suggested that E.adenophorum inhibited Bcl-2 expression, promoted Bax translocation and caspase-3, 9 activation in renal cells which means some pro-apoptotic genes expressed and apoptosis suppressor gene was inhibited, However, caspase-8 activity did not exhibit significant changes.The findings indicate that E.adenophorum probably has dose-dependent nephrotoxicity effect on goat as it markedly induced apoptosis.
Eupatorium adenophorum apoptosis qRT-PCR flow cytometry Saanen goat renal cell
HE Yajun REN Zhihua ZUO Zhicai DENG Junliang PENG Guangneng HE Wei WEI Yahui CHEN Weihong HU Yanchun LUO Biao WU Lei QIAO Yan MO Quan XU Ruiguang ZHOU Yancheng
Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicin Key Laboratory of Resource Biology and Biotechnology in Western China, School of Life Science, North
国内会议
中国畜牧兽医学会兽医内科与临床诊疗学分会第八届代表大会暨学术研讨会
大庆
英文
82-90
2015-07-22（万方平台首次上网日期，不代表论文的发表时间）