Effects of chromic chloride on chick embryo fibroblast viability
The objective of this study is to evaluate the effects of chromic chloride (CrCl3) on chick embryo fibroblast (CEF) viability.The cells were incubated with CrCl3 (0.02, 0.1, 0.5, 2.5, 12.5, and 62.5 μmol/L), and the viability was determined using MTT assay, morphological detection and flow cytometry.The results showed that lower concentrations of CrCl3 (0.02, 0.1, and 0.5 μmol/L) did not damage CEF viability.At 0.1 μmol/L, CrCl3 could increase CEF viability (P<0.05).However, at higher concentrations of CrCl3 (2.5, 12.5, and 62.5 μmol/L), the number of apoptotic and necrotic cells (P<0.01) and intracellular reactive oxygen species (P<0.01) increased.In addition, decreased mitochondrial membrane potential (P<0.01) and enhanced intracellular calcium levels (P<0.01) were observed after the exposure.Moreover, apoptotic morphological changes induced by these processes in CEF were confirmed using Hoechst 33258 staining.Cell death induced by higher concentrations of CrCl3 was caused by an apoptotic and a necrotic mechanism, whereas the main mechanism of oxidative stress and induced mitochondrial dysfunction was apoptotic death.The induced apoptotic death in CEF is concentration-and time-dependent.
chromium chloride chick embryo fibroblast oxidative stress apoptosis flow cytometry
LIU Mingchao LIU Jianzhu CHAI Tongjie
College of Veterinary Medicine, Research Center for Animal Disease Control Engineering Shandong Province, Shandong Agricultural University, Tai”an 271018,P.R.China
国内会议
中国畜牧兽医学会兽医内科与临床诊疗学分会第八届代表大会暨学术研讨会
大庆
英文
110-119
2015-07-22（万方平台首次上网日期，不代表论文的发表时间）