从不同视角探索糖尿病心肌病关键基因的系统生物学研究
Diabetic cardiomyopathy (DCM) is cardiac disease caused by diabetes but independent of hypertension, coronary artery disease (CAD), with high incidence, great harm, poor prognosis.Studies on DCM in recent decades shown that several factors such as metabolic disturbance, small vessel disease, myocardial fibrosis, cardiac autonomic dysfunction, and insulin resistance have been implicated as putative mechanisms leading to DCM.However, that pathological mechanisms of DCM on the molecule level is far from been fully understood, due to the complicity of DCM. Our current study means to characterize the pathophysiological mechanisms of DCM, and the pharmacological mechanisms of a particular herbal medicine (effective in treating DCM).We successfully build an STZ-induced DCM rat model, and then we collect microarray data and some physiological data from four groups of samples: Normal, Disease, Disease with herbal-medicine-treating and Disease with chemical-medicine-treating. After raw-data processing, we combined different approaches to comprehensively interpret the data: identify genes showing differential expression, or different connectivity, or strongly correlated to physiological character; construct weighted gene co-expression networks and determine significant modules. Gene-set enrichment analysis, pathway and PPI network mapping were also done. As results, besides verifying some mechanisms already known being related to DCM,We also identified several novel pathways and genes, and targets of that herbal medicine. All these results have implications in providing guidance and advice on pathological analysis and drug development of DCM.
糖尿病心肌病 病理机制 基因表达 系统生物学
孙之荣 李宇超
清华大学生命科学学院生物信息学教育部重点实验室
国内会议
第六届全国生物信息学与系统生物学学术大会暨国际生物信息学前沿研讨会
南京
中文
160-160
2014-10-06（万方平台首次上网日期，不代表论文的发表时间）