Simple generation of albino C57BL/6J mice with G291T mutation in the tyrosinase gene by the CRISPR/Cas9 system

摘要：

Gene knockout (KO) mice produced by gene targeting methods are essential tools for investigating endogenous gene function.In such targeting methods, genetic mutations are introduced by spontaneous homologous recombination in mouse embryonic stem cells (mESc).Gene KO mice are generated by the production of germline chimeras derived from specific gene-mutated mESc.Although reliable, this method is laborious, costly, and time consuming.In contrast, genome editing with engineered DNA nucleases induces site-directed gene mutations by direct injection of DNA or RNA of site-specific engineered nucleases into fertilized oocytes.It has been reported that 3 kinds of engineered nucleases (ZFN, TALENs and CRISPR/Cas) are useful for producing knockout (KO) mice.Therefore, these new technologies are expected to be useful for producing gene-modified animals.

作者: Seiya Mizuno

作者单位: Laboratory Animal Resource Center,University of Tsukuba,Japan

会议类型: 国内会议

会议名称: 2014第十一届中国实验动物科学年会

会议地点: 长春

会议语种:英文

页码: 145-146

在线出版日期: 2014-06-25（万方平台首次上网日期，不代表论文的发表时间）