Applications of a multifunction isolator system for the care of medium-sized laboratory animals harboring infectious diseases
The isolator system, including the transfer chain and disinfection chain, is designed and established according to national accreditation requirements and the related technology standards and experimental processes for performing animal research.It enables general detection and identification of high-risk pathogens in high-level biosafety laboratories to support our mission to improve the public health through biomedical research.The technology performance parameters of isolator system are empirically analyzed.The double port transfer exchange (DPTE) system is commonly used to transfer experimental materials, including bedding changes, food preparation, routine cage cleaning, animal testing, and waste disposal, and to keep laboratory animals in containment micro-environment.The open-front negative pressure cabinet is used as a transfer station to monitor the material flow safety among the isolators and other related security equipments.Peracetic acid, hydrogen peroxide and their derivatives are applied in different sterilizers to decontaminate the different types of isolators and maintain the performance of the internal isolator.A successful technology system is established to provide morphological screening, fluorescence microscopy immunoassay, laboratory animal breeding and immunization, and challenge and toxicity assays of the pathogen.The technology system meets the requirements of rapid detection and identification of pathogens based on the lethal infection, pathogenicity, and biological characteristics of the isolated pathogen.The multi-functional isolation chamber system offers the best possible safety and security to prevent pathogen contamination and enables efficient detection in a high-level biosafety laboratory.It is a safety protection hardware support system for working with infectious medium-sized laboratory animals.
Infection control Animal testing Isolator Biological safety
Xin Pan Jialin Cai Wei Fang Min Chen Wanqing Liao Yanguo Zhang Yi Li Ming Long Xiao Chen Siliang Zeng Boju Pan Zhonglei Pan Guanghui Zeng
Second Military Medical University, Shanghai 200433, China;Tongji University School of Medicine, Sha Second Military Medical University, Shanghai 200433, China National Center for Quality Supervision and Test of Building Engineering, Beijing 100013, China Shanghai Top Leader Medphparma Technologies Company Limited, Shanghai 200042, China
国内会议
北京
英文
29-40
2013-10-10（万方平台首次上网日期，不代表论文的发表时间）