The role of prolyl 4-hydroxylase expressed in non-small cell lung cancer based on an investigative result of traditional Chinese medicine treating lung carcinoma
Objectives:To investigate the expression of prolyl 4-hydroxylase(P4HB)in lung carcinoma and the intervention effect of Yiqi Chutan Formula(YQCTF).Methods:Lung carcinoma model was established by subcutaneously inoculating LEWIS lung carcinoma cells in C57BL/6J mice.The expressed differentially protein spots information of YQCTF(3.0 g/kg/d,gavage,daily,21 days)and model group were acquired by a two-way fluorescence difference gelelectrophoresis(2D-DIGE),and identified by matrix-assisted laser desorption ionization time of flight mass spectrometry(MALDI-TOF/TOF-MS).The differences in protein expression of P4HB was verified by Western blotting.Test the expression of P4HB by the application of clinical pathology and tissue microarray to obtain the clinical validation.Cultured A549 cells,divided into 15%serum containing of YQCTF and 15%serum combined DDP 1.5μg/ml group,cellular immunohistochemistry and RT-PCR detected the expression of P4HB and P4HBmRNA.Results:The proteomics research discovered that one-third of differential proteins which include P4HB were unfolded protein response(UPR)or related proteins were decreased in the YQCTF group(p<0.01).Clinical pathology and tissue microarray studies showed that P4HB expression in lung cancer tissue was stronger than adjacent tissues and normal lung epithelial(p<0.01).In the YQCTF and DDP combined group,the expression of P4HB and P4HBmRNA of A549 cell were decreased significantly(p <0.01).Conclusion:YQCTF could inhibit the LEWIS lung carcinoma”s growth,decrease the expression of P4HB in LEWIS lung carcinoma and A549 cells.YQCTF might take effect through regulating UPR in endoplasmic reticulum to inhibit the incidence and growth process of lung carcinoma.
Yiqi Chutan Formular (YQCTF) prolyl 4-hydroxylase beta polypeptide(P4HB) unfolded protein response(UPR)
Shumei-Wang Lizhu-Lin Shaoquan-Xiong Jingxu-Zhou
Department of the Clinical Teaching and Research College of Traditional Chinese Medicine Chongqing M Department of Oncology of the First Affiliated Hospital of GuangZhou Traditional Chinese Medicine Un Department of Oncology of the Teaching Hospital of Chengdu University of Traditional Chinese Medicin
国内会议
北京
英文
351-367
2015-07-01（万方平台首次上网日期，不代表论文的发表时间）