海藻糖对胰岛素干燥保护的分子动力学模拟
Biopharmaceuticals are proteins with a crucial role in the treatment of many diseases. However, these protein medicines are often thermally labile and therefore unsuitable for long-term application and storage, as they tend to lose their activity under ambient conditions. Desiccation is one approach to improving protein stability, but the drying process itself can cause irreversible damage. In the current study, insulin was chosen as an example of a thermally sensitive biopharmaceutical to investigate whether the disaccharide, trehalose, can prevent loss of structural integrity due to drying. The experiment was performed using replica exchange molecular simulation and Gromacs software with a Gromos96 (53a6) force field. The results indicate that trehalose preserves the bioactive structure of insulin during drying, consistent with the use of trehalose as a protectant for thermally sensitive biopharmaceuticals. For instance, at the water content of 1.77％, insulin without any protectants yields the highest RMSD value as 0.451 nm, then the RMSD of insulin in presence of trehalose only ca. 0.100 nm.
热敏感药物 胰岛素 海藻糖 干燥保护剂 分子动力学
刘立 李代禧 余华星 潘琦 柴培 王丹丹 郭柏松 杨春生 刘宝林
上海理工大学食品科学与工程研究所,上海,200093 上海东富龙科技股份有限公司,上海,201108
国内会议
苏州
中文
1-22
2014-10-26（万方平台首次上网日期，不代表论文的发表时间）