A Single Chain Antibody using LoxP511 as the Linker Enables Large-Content Phage Library Construction via Cre/LoxP Recombination

摘要：

　　To obtain natural or ”me-better” antibodies (e.g., affinity-maturated antibodies), phage display libraries are widely used.However, the likelihood of obtaining satisfactory antibodies depends on the library content.Here, we used computer-aided design to model the use of the LoxPS11 site as a linker between the heavy and light variable domains of an antibody for construction of a large single-chain fragment (scFv) antibody phage library by using the Cre/LoxP recombinant system.

关键词： scFv large-content library linker Cre/LoxP recombinant

作者: Yan Zhang Chunxia Qiao Jiannan Feng Wei Wang Ming Lv Zhou Lin Jing Geng Yali Li Beifen Shen Yuanfang Ma Yan Li

作者单位: Department of Gynecology and Obstetrics, PLA General Hospital, Fuxing Road No.28, Beijing 100853, Ch Laboratory of Immunology, Institute of Basic Medical Sciences, Beijing 100850,China Laboratory of Immunology, Institute of Basic Medical Sciences, Beijing 100850,China;Laboratory of Ce Laboratory of Cellular and Molecular Immunology, Institute of Immunology, Henan University, Kaifeng,

会议类型: 国内会议

会议名称: 第七届国际分子模拟与信息技术应用学术会议

会议地点: 苏州

会议语种:英文

页码: 31-57

在线出版日期: 2014-10-26（万方平台首次上网日期，不代表论文的发表时间）