A Rapid and Simple Method for Detection of Tobacco mosaic virus in Nicotiana tabacum by Aqueous Extract
Tobacco mosaic virus (TMV) is a widespread plant virus of the genus Tobamovirus that infects plants,especially tobacco and other members of the family Solanaceae.The infection causes mottling and discoloration on the leaves.In this study we reported the simple and rapid method for detection of TMV based on aqueous extract and agarose gel electrophoresis, and all procedures require 20 min.A small amount of tobacco leaves (~ 10 mg) was ground in sterile water (~ 100 μL), and the electrophoretic bands of about 6000 nt in aqueous extract were detected through agarose gel electrophoresis.The Northern-Blot analysis targeting TMV-replicase gene showed that the electrophoretic bands was TMV genomic.However, the aqueous extract is unstable under room temperature and degraded after 6 hr extraction.Therefore, this method is more rapid, simple and economic than those methods reported previously for a large number of samles.
Tobacco mosaic virus (TMV) Detection Aqueous extract
Jinguang Yang Fenglong Wang Lili Shen Yumei Qian Dexin Chen
Key Laboratory of Tobacco Pest Monitoring Controlling & Integrated Management, State Tobacco Monopoly Bureau, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, Shandong 266101
国内会议
厦门
英文
338-343
2010-07-01（万方平台首次上网日期，不代表论文的发表时间）