De-novo assembly and characterization of Chlorella minutissima UTEX2341 transcriptome by paired-end sequencing
Chlorella minutissima is considered as one promising feedstock for biofuels in the future.Here,the transcriptome from oil-rich strain UTEX2341 of C.minutissima was generated based on Illumina paired-end sequencing.Through de-novo assembly,14,905 total isogenes was obtained and compacted into 6,216 unigenes.80%of unigenes were assigned with GO terms and further subdivided into 55 sub-categories.KEGG analysis showed that 37.2%of unigenes could be accessed and mapped into 278 pathways.Interestingly,genes encoding key enzymes involved in biosynthesis,elongation,and metabolism of fatty acids were identified,including malonyl-CoA-ACP transacylase,3-ketoacyl-ACP synthase,3-ketoacyl-ACP reductase,and others.Moreover,the genes involved in triacylglycerol (TAG) biosynthesis and metabolism also were found.Therefore,the transcriptome analysis of C.minutissima UTEX2341 not only supplies a comprehensive insight into the molecular pathway involved in biosynthesis of biofuels precursors,but also provides substantial valuable genomic resources to accelerate further development and utilization of biofuels.
Chlorella minutissima UTEX2341 transcriptome paired-end sequencing fatty acids TAG
Mingjia Yu Xiangzhi Lin Shanjun Yang Zhaokai Wang Yang Zhang
Engineering Research Center of Marine Biological Resource Comprehensive Utilization,Third Institute Key Laboratory of Tropical Marine Bio-resources and Ecology,South China Sea Institute of Oceanology,
国内会议
福建省海洋学会2014年学术年会暨福建省科协第十四届学术年会分会场
福建平潭
英文
140-148
2014-09-01（万方平台首次上网日期，不代表论文的发表时间）