Diversity and novelty of actinobacteria in Arctic marine sediments
The actinobacterial diversity of Arctic marine sediments was investigated using culture-dependent and culture-independent approaches.A total of 152 strains were isolated from seven different media; 18 isolates were selected for phylogenetic analysis on the basis of their 16S rRNA gene sequences.Results showed that the 18 isolates belonged to a potential novel genus and 10 known genera including Actinotalea,Arthrobacter,Brachybacterium,Brevibacterium,Kocuria,Kytococcus,Microbacterium,Micrococcus,Mycobacterium,and Pseudonocardia.Subsequently,172 rDNA clones were selected by restriction fragment length polymorphism (RFLP) analysis from 692 positive clones within four action bacteria-specific 16S rDNA libraries of Arctic marine sediments,and then these 172 clones were sequenced.In total,67 phylotypes were clustered in 11 known genera of actinobacteria including Agrococcus,Cellulomonas,Demequina,Iamia,Ilumatobacter,Janibacter,Kocuria,Microbacterium,Phycicoccus,Propionibacterium,and Pseudonocardia,along with other,unidentified actinobacterial clones.Based on the detection of a substantial number of uncultured phylotypes showing low BLAST identities (<95%),this study confirms that Arctic marine environments harbour highly diverse actinobacterial communities,many of which appear to be novel,uncultured species.
Actinobacteria Arctic diversity marine sediment
Gaiyun Zhang Tingfeng Cao Jianxi Ying Yanliu Yang Lingqi Ma
Key Laboratory of Marine Biogenetic Resources,Third Institute of Oceanography,State Oceanic Administration,Xiamen 361005,P.R. China
国内会议
福建省海洋学会2014年学术年会暨福建省科协第十四届学术年会分会场
福建平潭
英文
200-215
2014-09-01（万方平台首次上网日期，不代表论文的发表时间）