Enrichment and characterization of ananaerobic cellulolytic microbial consortiumSQD-1.1 from mangrove soil
Enrichment of microbial consortia provides an approach to simulate and investigate microbial communities in natural environments.In this study,a cellulolytic microbial consortium SQD-1.1 was enriched from mangrove soil of Qinglan port (Hainan,China) by 27-times continuous subcultivation under anaerobic static conditions.The consortium could completely degrade0.2%(w/v) filter paper within three days and utilized it as the sole carbon source.PCR-denaturing gradient gel electrophoresis (DGGE) analysis revealed a stable microbial community structure in the incubation process of 10days and in the procedure of subcultivation.24 OTUs belonging to seven phyla were obtained from the full-length 16S rRNA gene library.Five clones,closest related to the genera Alkaliflexus,Clostridium,Alistipes,Spirochaeta and Trichococcus,were the predominant ones.Among them,M117,phylogeneticly showing high similarity (16S rRNA gene identity,95.3%) with the cellulolytic anaerobic bacterium Clostridium straminisolvens CSK1T,was the potential key cellulolytic bacterium.Using the plate cultivation method,twelve strains,including one potential new species and four potential new species of new genera,were isolated.The strainP2,corresponding to the most frequently detected clone (M05) in the 16S rRNA gene library,showed both CMCase and xylanase activity and may be another important cellulolytic bacterium.The findings of cellulase activity in cell pellet and cohesion and dockerin domains in metagenome data further suggested the potential of utilization of cellulosome by the consortium to degrade cellulose.Consortium SQD-1.1 provides a candidate for investigating the mechanism of cellulose degradation under anoxic conditions in natural environments.
microbial consortia cellulolytic bacterium mangrove cellulosome Cellulase
Zhao-Ming Gao Ling-Wei Ruan Xun Xu
State Key Laboratory of Marine Environmental Science,College of Ocean & Earth Science,Xiamen Univers Key Laboratory of Marine Biogenetic Resources,Third Institute of Oceanography,State Oceanic Administ
国内会议
福建省海洋学会2014年学术年会暨福建省科协第十四届学术年会分会场
福建平潭
英文
219-235
2014-09-01（万方平台首次上网日期，不代表论文的发表时间）