Isolation and characterization of five lytic bacteriophages infecting a Vibrio strain closely related to Vibrio owensii
Vibrio owensii is a potential bacterial pathogen in marine aquaculture system.In this study,five lytic phages specific against Vibrio strain B8D,closely related to V.owensii,were isolated from seawater of an abalone farm.The phages were characterized with respect to morphology,genome size,growth phenotype,as well as thermal and pH stability.All phages were found to belong to the family Siphoviridae with long non-contractile tails and terminal fibers.Restriction analysis indicated that the five phages were dsDNA viruses with molecular weights ranging from~30 kb to~48 kb.One step growth experiments revealed that the phages were heterogeneous in latent periods (10-70 min),rise periods (40-70 min),and burst sizes (23-331 plaque forming units (PFU)/infected cell) at the same host strain.All phages were thermal stable and were tolerant to a wide range of pH.The results indicated that these phages could be potential candidates of a phage cocktail for biological control of V.owensii in aquaculture systems.
lytic bacetriophages Siphoviridae biological control Vibrio owensii
Yan-Ping Yu Ting Gong Günter Jost Wen-Hua Liu De-Zan Ye Zhu-Hua Luo
Key Laboratory of Marine Biogenetic Resources,Third Institute of Oceanography,State Oceanic Administ Key Laboratory of Marine Biogenetic Resources,Third Institute of Oceanography,State Oceanic Administ Leibniz Institute for Baltic Sea Research Warnemünde,Rostock,Germany Marine Biology Institute,Shantou University,Shantou P R China
国内会议
福建省海洋学会2014年学术年会暨福建省科协第十四届学术年会分会场
福建平潭
英文
427-441
2014-09-01（万方平台首次上网日期，不代表论文的发表时间）